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An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila
We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH...
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| Veröffentlicht in: | eLife |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
eLife Sciences Publications, Ltd
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6095692/ https://ncbi.nlm.nih.gov/pubmed/30091705 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.38709 |
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