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A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells
Here, we describe a novel method based on intronic MiMIC insertions described in Nagarkar-Jaiswal et al. (2015) to perform conditional gene inactivation in Drosophila. Mosaic analysis in Drosophila cannot be easily performed in post-mitotic cells. We therefore, therefore, developed Flip-Flop, a flip...
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| Udgivet i: | eLife |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
eLife Sciences Publications, Ltd
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5493436/ https://ncbi.nlm.nih.gov/pubmed/28561736 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.26420 |
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