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Use of the CRISPR-Cas9 system in Drosophila cultured cells to introduce fluorescent tags into endogenous genes
The CRISPR-Cas9 system makes it possible to cause double-strand breaks in specific regions, inducing repair. In the presence of a donor construct, repair can involve insertion or ‘knock-in’ of an exogenous cassette. One common application of knock-in technology is to generate cell lines expressing f...
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| Publicado no: | Curr Protoc Mol Biol |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7213786/ https://ncbi.nlm.nih.gov/pubmed/31869524 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.112 |
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