An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

We previously reported a CRISPR-mediated knock-in strategy into introns of Drosophila genes, generating an attP-FRT-SA-T2A-GAL4-polyA-3XP3-EGFP-FRT-attP transgenic library for multiple uses (Lee et al., 2018a). The method relied on double stranded DNA (dsDNA) homology donors with ~1 kb homology arms...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Kanca, Oguz, Zirin, Jonathan, Garcia-Marques, Jorge, Knight, Shannon Marie, Yang-Zhou, Donghui, Amador, Gabriel, Chung, Hyunglok, Zuo, Zhongyuan, Ma, Liwen, He, Yuchun, Lin, Wen-Wen, Fang, Ying, Ge, Ming, Yamamoto, Shinya, Schulze, Karen L, Hu, Yanhui, Spradling, Allan C, Mohr, Stephanie E, Perrimon, Norbert, Bellen, Hugo J
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2019
Assuntos:
Genetics and Genomics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6855806/
https://ncbi.nlm.nih.gov/pubmed/31674908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.51539
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