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Endogenous C-terminal Tagging by CRISPR/Cas9 in Trypanosoma cruzi
To achieve the C-terminal tagging of endogenous proteins in T. cruzi we use the Cas9/pTREX-n vector ( Lander et al., 2015 ) to insert a specific tag sequence (3xHA or 3xc-Myc) at the 3’ end of a specific gene of interest (GOI). Chimeric sgRNA targeting the 3’ end of the GOI is PCR-amplified and clon...
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| Pubblicato in: | Bio Protoc |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Bio-Protocol
2017
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5531189/ https://ncbi.nlm.nih.gov/pubmed/28758140 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2299 |
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