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Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Rapid detection of nucleic acids is integral for clinical diagnostics and biotechnological applications. We recently developed a platform termed SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing) that combines isothermal pre-amplification with Cas13 to detect single molecules of RNA o...

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Publicat a:Science
Autors principals: Gootenberg, Jonathan S., Abudayyeh, Omar O., Kellner, Max J., Joung, Julia, Collins, James J., Zhang, Feng
Format: Artigo
Idioma:Inglês
Publicat: 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5961727/
https://ncbi.nlm.nih.gov/pubmed/29449508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aaq0179
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