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SHERLOCK: Nucleic acid detection with CRISPR nucleases

Rapid detection of nucleic acids is integral for applications in clinical diagnostics and biotechnology. We have recently established a CRISPR-based diagnostic platform that combines nucleic acid pre-amplification with CRISPR-Cas enzymology for specific recognition of desired DNA or RNA sequences. T...

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Détails bibliographiques
Publié dans:Nat Protoc
Auteurs principaux: Kellner, Max J., Koob, Jeremy, Gootenberg, Jonathan S., Abudayyeh, Omar O., Zhang, Feng
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6956564/
https://ncbi.nlm.nih.gov/pubmed/31548639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0210-2
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