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Crystal structures of thrombin in complex with chemically modified thrombin DNA aptamers reveal the origins of enhanced affinity

Thrombin-binding aptamer (TBA) is a DNA 15-mer of sequence 5′-GGT TGG TGT GGT TGG-3′ that folds into a G-quadruplex structure linked by two T-T loops located on one side and a T-G-T loop on the other. These loops are critical for post-SELEX modification to improve TBA target affinity. With this goal...

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Bibliografiset tiedot
Julkaisussa:Nucleic Acids Res
Päätekijät: Dolot, Rafal, Lam, Curtis H, Sierant, Malgorzata, Zhao, Qiang, Liu, Feng-Wu, Nawrot, Barbara, Egli, Martin, Yang, Xianbin
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 2018
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5961234/
https://ncbi.nlm.nih.gov/pubmed/29684204
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky268
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