Direct Detection of Thrombin Binding to 8-Bromodeoxyguanosine-Modified Aptamer: Effects of Modification on Affinity and Kinetics

The affinity of an 8-bromodeoxyguanosine- (8-BrdG-) substituted thrombin-binding aptamer (TBA-Br), which has the 1st and 10th guanosine residues replaced with 8-BrdG, was estimated using reflectometric interference spectroscopy (RIfS). When comparing TBA-Br with unmodified TBA (TBA-H), it was demons...

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Bibliographic Details
Main Authors: Goji, Shou, Matsui, Jun
Format: Artigo
Language:Inglês
Published: SAGE-Hindawi Access to Research 2011
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3175402/
https://ncbi.nlm.nih.gov/pubmed/21941628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2011/316079
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