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Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1
The RNA-guided Cpf1 nuclease cleaves double-stranded DNA targets complementary to the CRISPR RNA (crRNA), and has been harnessed for genome editing technologies. Recently, Acidaminococcus sp. BV3L6 (AsCpf1) was engineered to recognize altered DNA sequences as the protospacer adjacent motif (PAM), th...
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| Publicado no: | Mol Cell |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5957533/ https://ncbi.nlm.nih.gov/pubmed/28595896 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2017.04.019 |
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