Crystal structure of Cpf1 in complex with guide RNA and target DNA

Cpf1 is an RNA-guided endonuclease of a type V CRISPR-Cas system that has been recently harnessed for genome editing. Here, we report the crystal structure of Acidaminococcus sp. Cpf1 (AsCpf1) in complex with the guide RNA and its target DNA, at 2.8 Å resolution. AsCpf1 adopts a bilobed architecture...

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Detalhes bibliográficos
Publicado no:Cell
Main Authors: Yamano, Takashi, Nishimasu, Hiroshi, Zetsche, Bernd, Hirano, Hisato, Slaymaker, Ian M., Li, Yinqing, Fedorova, Iana, Nakane, Takanori, Makarova, Kira S., Koonin, Eugene V., Ishitani, Ryuichiro, Zhang, Feng, Nureki, Osamu
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4899970/
https://ncbi.nlm.nih.gov/pubmed/27114038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2016.04.003
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