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Structural basis for the canonical and non-canonical PAM recognition by CRISPR-Cpf1
The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA), and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic ge...
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| Publicado no: | Mol Cell |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5957536/ https://ncbi.nlm.nih.gov/pubmed/28781234 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2017.06.035 |
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