Structural basis for the canonical and non-canonical PAM recognition by CRISPR-Cpf1

The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA), and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic ge...

Full description

Saved in:
Bibliographic Details
Published in:Mol Cell
Main Authors: Yamano, Takashi, Zetsche, Bernd, Ishitani, Ryuichiro, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Format: Artigo
Language:Inglês
Published: 2017
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5957536/
https://ncbi.nlm.nih.gov/pubmed/28781234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2017.06.035
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items