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Structural basis for the canonical and non-canonical PAM recognition by CRISPR-Cpf1

The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA), and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic ge...

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Dades bibliogràfiques
Publicat a:Mol Cell
Autors principals: Yamano, Takashi, Zetsche, Bernd, Ishitani, Ryuichiro, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Format: Artigo
Idioma:Inglês
Publicat: 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5957536/
https://ncbi.nlm.nih.gov/pubmed/28781234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2017.06.035
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