Engineered Cpf1 variants with altered PAM specificities increase genome targeting range

The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells(1–7). However, the utility of the commonly used Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1) and Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1) is limited by their requirement of a TTTV protospacer adjacent motif...

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Publicado en:Nat Biotechnol
Main Authors: Gao, Linyi, Cox, David B.T., Yan, Winston X., Manteiga, John C., Schneider, Martin W., Yamano, Takashi, Nishimasu, Hiroshi, Nureki, Osamu, Crosetto, Nicola, Zhang, Feng
Formato: Artigo
Idioma:Inglês
Publicado: 2017
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5548640/
https://ncbi.nlm.nih.gov/pubmed/28581492
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3900
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