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Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses

The combination of yeast surface display and flow cytometric analyses and selections is being used with increasing frequency to alter specificity of macromolecular recognition, including both protein–protein and protein–nucleic acid interactions. Here we describe the use of yeast surface display and...

詳細記述

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書誌詳細
出版年:Protein Eng Des Sel
主要な著者: Niyonzima, Nixon, Lambert, Abigail R., Werther, Rachel, De Silva Feelixge, Harshana, Roychoudhury, Pavitra, Greninger, Alexander L., Stone, Daniel, Stoddard, Barry L., Jerome, Keith R.
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 2017
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5914421/
https://ncbi.nlm.nih.gov/pubmed/28873986
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzx037
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