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Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses
The combination of yeast surface display and flow cytometric analyses and selections is being used with increasing frequency to alter specificity of macromolecular recognition, including both protein–protein and protein–nucleic acid interactions. Here we describe the use of yeast surface display and...
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| 出版年: | Protein Eng Des Sel |
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| 主要な著者: | , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2017
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5914421/ https://ncbi.nlm.nih.gov/pubmed/28873986 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzx037 |
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