Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses

The combination of yeast surface display and flow cytometric analyses and selections is being used with increasing frequency to alter specificity of macromolecular recognition, including both protein–protein and protein–nucleic acid interactions. Here we describe the use of yeast surface display and...

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Библиографические подробности
Опубликовано в: :Protein Eng Des Sel
Главные авторы: Niyonzima, Nixon, Lambert, Abigail R., Werther, Rachel, De Silva Feelixge, Harshana, Roychoudhury, Pavitra, Greninger, Alexander L., Stone, Daniel, Stoddard, Barry L., Jerome, Keith R.
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2017
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Original Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5914421/
https://ncbi.nlm.nih.gov/pubmed/28873986
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzx037
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