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Evolving Artificial Metalloenzymes via Random Mutagenesis

Random mutagenesis has the potential to optimize the efficiency and selectivity of protein catalysts without requiring detailed knowledge of protein structure; however, introducing synthetic metal cofactors complicates the expression and screening of enzyme libraries, and activity arising from free...

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Bibliografske podrobnosti
izdano v:Nat Chem
Main Authors: Yang, Hao, Swartz, Alan M., Park, Hyun June, Srivastava, Poonam, Ellis-Guardiola, Ken, Upp, David M., Lee, Gihoon, Belsare, Ketaki, Gu, Yifan, Zhang, Chen, Moellering, Raymond E., Lewis, Jared C.
Format: Artigo
Jezik:Inglês
Izdano: 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5891097/
https://ncbi.nlm.nih.gov/pubmed/29461523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchem.2927
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