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Chemo-enzymatic synthesis of selectively (13)C/(15)N-labeled RNA for NMR structural and dynamics studies

RNAs are an important class of cellular regulatory elements, and they are well characterized by X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy in their folded or bound states. However, the apo or unfolded states are more difficult to characterize by either method. Particular...

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Publicat a:Methods Enzymol
Autors principals: Alvarado, Luigi J., Longhini, Andrew P., LeBlanc, Regan M., Chen, Bin, Kreutz, Christoph, Dayie, T. Kwaku
Format: Artigo
Idioma:Inglês
Publicat: 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5879494/
https://ncbi.nlm.nih.gov/pubmed/25432748
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-801122-5.00007-6
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