NMR of enzymatically synthesized uniformly 13C15N-labeled DNA oligonucleotides.

A procedure for the enzymatic synthesis of uniformly 13C15N-labeled DNA oligonucleotides in milligram quantities for NMR studies is described. Deoxynucleotides obtained from microorganisms grown on 13C and 15N nutrient sources are enzymatically phosphorylated to dNTPs, and the dNTPs are incorporated...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Zimmer, D P, Crothers, D M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42110/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7724521/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.8.3091
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