Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations

Stable isotope labeling is central to NMR studies of nucleic acids. Development of methods that incorporate labels at specific atomic positions within each nucleotide promises to expand the size range of RNAs that can be studied by NMR. Using recombinantly expressed enzymes and chemically synthesize...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Longhini, Andrew P., LeBlanc, Regan M., Becette, Owen, Salguero, Carolina, Wunderlich, Christoph H., Johnson, Bruce A., D'Souza, Victoria M., Kreutz, Christoph, Dayie, T. Kwaku
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2016
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4824079/
https://ncbi.nlm.nih.gov/pubmed/26657632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1333
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