Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations

Stable isotope labeling is central to NMR studies of nucleic acids. Development of methods that incorporate labels at specific atomic positions within each nucleotide promises to expand the size range of RNAs that can be studied by NMR. Using recombinantly expressed enzymes and chemically synthesize...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Longhini, Andrew P., LeBlanc, Regan M., Becette, Owen, Salguero, Carolina, Wunderlich, Christoph H., Johnson, Bruce A., D'Souza, Victoria M., Kreutz, Christoph, Dayie, T. Kwaku
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2016
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4824079/
https://ncbi.nlm.nih.gov/pubmed/26657632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1333
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