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Development of an efficient E. coli expression and purification system for a catalytically active, human Cullin3-RINGBox1 protein complex and elucidation of its quaternary structure with Keap1
The Cullin3-based E3 ubiquitin ligase complex is thought to play an important role in the cellular response to oxidative stress and xenobiotic assault. While limited biochemical studies of the ligase’s role in these complex signaling pathways are beginning to emerge, structural studies are lagging f...
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| Udgivet i: | Biochem Biophys Res Commun |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5868348/ https://ncbi.nlm.nih.gov/pubmed/20735986 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.08.062 |
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