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Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso(®) Solubility and Expression Screening System

The simplicity, speed, and low cost of bacterial culture make E. coli the system of choice for most initial trials of recombinant protein expression. However, many heterologous proteins are either poorly expressed in bacteria, or are produced as incorrectly folded, insoluble aggregates that lack the...

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Bibliografski detalji
Izdano u:Curr Protoc Protein Sci
Glavni autori: Steinmetz, Eric, Auldridge, Michele
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5791521/
https://ncbi.nlm.nih.gov/pubmed/29091274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.39
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