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Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso(®) Solubility and Expression Screening System

The simplicity, speed, and low cost of bacterial culture make E. coli the system of choice for most initial trials of recombinant protein expression. However, many heterologous proteins are either poorly expressed in bacteria, or are produced as incorrectly folded, insoluble aggregates that lack the...

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Dades bibliogràfiques
Publicat a:Curr Protoc Protein Sci
Autors principals: Steinmetz, Eric, Auldridge, Michele
Format: Artigo
Idioma:Inglês
Publicat: 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5791521/
https://ncbi.nlm.nih.gov/pubmed/29091274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.39
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