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Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli
The enteric bacterium Escherichia coli is the most extensively used prokaryotic organism for production of proteins of therapeutic or commercial interest. However, it is common that heterologous over-expressed recombinant proteins fail to properly fold resulting in formation of insoluble aggregates...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2683908/ https://ncbi.nlm.nih.gov/pubmed/19328840 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gene.2009.03.011 |
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