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Effect of Chemical Chaperones in Improving the Solubility of Recombinant Proteins in Escherichia coli
The recovery of active proteins from inclusion bodies usually involves chaotrope-induced denaturation, followed by refolding of the unfolded protein. The efficiency of renaturation is low, leading to reduced yield of the final product. In this work, we report that recombinant proteins can be overexp...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3127727/ https://ncbi.nlm.nih.gov/pubmed/21551288 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.05259-11 |
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