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Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso(®) Solubility and Expression Screening System
The simplicity, speed, and low cost of bacterial culture make E. coli the system of choice for most initial trials of recombinant protein expression. However, many heterologous proteins are either poorly expressed in bacteria, or are produced as incorrectly folded, insoluble aggregates that lack the...
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| izdano v: | Curr Protoc Protein Sci |
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| Main Authors: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2017
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5791521/ https://ncbi.nlm.nih.gov/pubmed/29091274 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.39 |
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