γ-Secretase Epsilon-cleavage Assay

γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay ( Kang et al., 2015 ), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are HEK293 cells with a stably integrated luciferase reporter und...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Xu, Ting-Hai, Yan, Yan, Harikumar, Kaleeckal G., Miller, Laurence J., Melcher, Karsten, Xu, Eric H.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2017
Fag:
Methods Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5790314/
https://ncbi.nlm.nih.gov/pubmed/29392164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2900
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