Detection of Membrane Protein Interactions by Cell-based Tango Assays

The Tango assay is a protein-protein interaction assay, in which a transcription factor (rTA) is fused to a membrane-bound protein via a linker that contains a cleavage site for TEV protease, whereas a soluble interaction partner is fused to TEV protease ( Barnea et al., 2008 ). Association between...

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Udgivet i:Bio Protoc
Main Authors: Yan, Yan, Xu, Ting-Hai, Harikumar, Kaleeckal G., Miller, Laurence J., Melcher, Karsten, Xu, Eric H.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2017
Fag:
Methods Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5790315/
https://ncbi.nlm.nih.gov/pubmed/29392165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2903
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