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Detection of Membrane Protein Interactions by Cell-based Tango Assays
The Tango assay is a protein-protein interaction assay, in which a transcription factor (rTA) is fused to a membrane-bound protein via a linker that contains a cleavage site for TEV protease, whereas a soluble interaction partner is fused to TEV protease ( Barnea et al., 2008 ). Association between...
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| Publicado en: | Bio Protoc |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Bio-Protocol
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5790315/ https://ncbi.nlm.nih.gov/pubmed/29392165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2903 |
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