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γ-Secretase Epsilon-cleavage Assay
γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay ( Kang et al., 2015 ), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are HEK293 cells with a stably integrated luciferase reporter und...
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| Vydáno v: | Bio Protoc |
|---|---|
| Hlavní autoři: | , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Bio-Protocol
2017
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5790314/ https://ncbi.nlm.nih.gov/pubmed/29392164 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2900 |
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