Improved in Vitro Folding of the Y(2) G Protein-Coupled Receptor into Bicelles

Prerequisite for structural studies on G protein-coupled receptors is the preparation of highly concentrated, stable, and biologically active receptor samples in milligram amounts of protein. Here, we present an improved protocol for Escherichia coli expression, functional refolding, and reconstitut...

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Detalhes bibliográficos
Publicado no:Front Mol Biosci
Main Authors: Schmidt, Peter, Bender, Brian J., Kaiser, Anette, Gulati, Khushboo, Scheidt, Holger A., Hamm, Heidi E., Meiler, Jens, Beck-Sickinger, Annette G., Huster, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018
Assuntos:
Molecular Biosciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5776092/
https://ncbi.nlm.nih.gov/pubmed/29387686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmolb.2017.00100
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