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Expression, Functional Characterization, and Solid-State NMR Investigation of the G Protein-Coupled GHS Receptor in Bilayer Membranes

The expression, functional reconstitution and first NMR characterization of the human growth hormone secretagogue (GHS) receptor reconstituted into either DMPC or POPC membranes is described. The receptor was expressed in E. coli. refolded, and reconstituted into bilayer membranes. The molecule was...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Schrottke, Stefanie, Kaiser, Anette, Vortmeier, Gerrit, Els-Heindl, Sylvia, Worm, Dennis, Bosse, Mathias, Schmidt, Peter, Scheidt, Holger A., Beck-Sickinger, Annette G., Huster, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5384189/
https://ncbi.nlm.nih.gov/pubmed/28387359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep46128
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