Improved in Vitro Folding of the Y(2) G Protein-Coupled Receptor into Bicelles

Prerequisite for structural studies on G protein-coupled receptors is the preparation of highly concentrated, stable, and biologically active receptor samples in milligram amounts of protein. Here, we present an improved protocol for Escherichia coli expression, functional refolding, and reconstitut...

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Bibliografske podrobnosti
izdano v:Front Mol Biosci
Main Authors: Schmidt, Peter, Bender, Brian J., Kaiser, Anette, Gulati, Khushboo, Scheidt, Holger A., Hamm, Heidi E., Meiler, Jens, Beck-Sickinger, Annette G., Huster, Daniel
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2018
Teme:
Molecular Biosciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5776092/
https://ncbi.nlm.nih.gov/pubmed/29387686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmolb.2017.00100
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