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Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons

Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell li...

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Bibliografische gegevens
Gepubliceerd in:Bio Protoc
Hoofdauteurs: Trajkovic, Katarina, Jeong, Hyunkyung, Krainc, Dimitri
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Bio-Protocol 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5759345/
https://ncbi.nlm.nih.gov/pubmed/29326963
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2675
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