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Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

DNA double-strand breaks (DSB) are serious DNA lesions. Analysis of the formation and repair of DSB is relevant in a broad spectrum of research areas including genome integrity, genotoxicity, radiation biology, aging, cancer, and drug development. In response to DSB, the histone H2AX is phosphorylat...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Popp, Henning D., Brendel, Susanne, Hofmann, Wolf-Karsten, Fabarius, Alice
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755291/
https://ncbi.nlm.nih.gov/pubmed/29155797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56617
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