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Prediction of antibody structural epitopes via random peptide library screening and next generation sequencing

Next generation sequencing (NGS) is widely applied in immunological research, but has yet to become common in antibody epitope mapping. A method utilizing a 12-mer random peptide library expressed in bacteria coupled with magnetic-based cell sorting and NGS correctly identified more than 75% of epit...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:J Immunol Methods
Päätekijät: Ibsen, Kelly N., Daugherty, Patrick S.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2017
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5698135/
https://ncbi.nlm.nih.gov/pubmed/28827189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2017.08.004
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