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Prediction of antibody structural epitopes via random peptide library screening and next generation sequencing

Next generation sequencing (NGS) is widely applied in immunological research, but has yet to become common in antibody epitope mapping. A method utilizing a 12-mer random peptide library expressed in bacteria coupled with magnetic-based cell sorting and NGS correctly identified more than 75% of epit...

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Detalhes bibliográficos
Publicado no:J Immunol Methods
Main Authors: Ibsen, Kelly N., Daugherty, Patrick S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5698135/
https://ncbi.nlm.nih.gov/pubmed/28827189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2017.08.004
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