CRISPR/Cas9 screening using unique molecular identifiers

Loss‐of‐function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as...

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Bibliographic Details
Published in:Mol Syst Biol
Main Authors: Schmierer, Bernhard, Botla, Sandeep K, Zhang, Jilin, Turunen, Mikko, Kivioja, Teemu, Taipale, Jussi
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2017
Subjects:
Method
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5658704/
https://ncbi.nlm.nih.gov/pubmed/28993443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20177834
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