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CRISPR/Cas9 screening using unique molecular identifiers
Loss‐of‐function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as...
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| Publicado no: | Mol Syst Biol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5658704/ https://ncbi.nlm.nih.gov/pubmed/28993443 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20177834 |
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