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Engineering specificity from broad to narrow: Design of a BLIP variant that exclusively binds and detects KPC β-lactamase
The β-lactamase inhibitory protein (BLIP) binds and inhibits a wide range of class A β-lactamases including the TEM-1 β-lactamase (K(i)= 0.5 nM), which is widely present in Gram-negative bacteria, and the KPC-2 β-lactamase (K(i)= 1.2 nM), which hydrolyzes virtually all clinically useful β-lactam ant...
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| Publicado no: | ACS Infect Dis |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5656002/ https://ncbi.nlm.nih.gov/pubmed/27756125 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsinfecdis.6b00160 |
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