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Fluorescence polarization assays to measure interactions between Gα subunits of heterotrimeric G proteins and regulatory motifs
Fluorescence polarization (FP) is a simple and sensitive method allowing for the quantification of interactions between proteins and fluorescently-tagged small molecules like peptides. Heterotrimeric G proteins are critical signal transducing molecules and their activity is controlled by a complex n...
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| Опубликовано в: : | Methods Cell Biol |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2017
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5654624/ https://ncbi.nlm.nih.gov/pubmed/28964332 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2017.07.007 |
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