A Homogeneous Method to Measure Nucleotide Exchange by α-Subunits of Heterotrimeric G-Proteins Using Fluorescence Polarization

The mainstay of assessing guanosine diphosphate release by the α-subunit of a heterotrimeric G-protein is the [(35)S]guanosine 5′-O-(3-thiotriphosphate) (GTPγS) radionucleotide-binding assay. This assay requires separation of protein-bound GTPγS from free GTPγS at multiple time points followed by qu...

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Detaylı Bibliyografya
Asıl Yazarlar: Muller, Robin E., Klein, Klara R., Hutsell, Stephanie Q., Siderovski, David P., Kimple, Adam J.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Mary Ann Liebert, Inc. 2010
Konular:
Technical Briefs
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957273/
https://ncbi.nlm.nih.gov/pubmed/20662737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/adt.2010.0286
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