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A Homogeneous Method to Measure Nucleotide Exchange by α-Subunits of Heterotrimeric G-Proteins Using Fluorescence Polarization
The mainstay of assessing guanosine diphosphate release by the α-subunit of a heterotrimeric G-protein is the [(35)S]guanosine 5′-O-(3-thiotriphosphate) (GTPγS) radionucleotide-binding assay. This assay requires separation of protein-bound GTPγS from free GTPγS at multiple time points followed by qu...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Mary Ann Liebert, Inc.
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2957273/ https://ncbi.nlm.nih.gov/pubmed/20662737 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/adt.2010.0286 |
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