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Fluorescence polarization assays to measure interactions between Gα subunits of heterotrimeric G proteins and regulatory motifs

Fluorescence polarization (FP) is a simple and sensitive method allowing for the quantification of interactions between proteins and fluorescently-tagged small molecules like peptides. Heterotrimeric G proteins are critical signal transducing molecules and their activity is controlled by a complex n...

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Vydáno v:Methods Cell Biol
Hlavní autoři: Maziarz, Marcin, Garcia-Marcos, Mikel
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5654624/
https://ncbi.nlm.nih.gov/pubmed/28964332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2017.07.007
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