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RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus

RNase H1-dependent antisense oligonucleotides (ASOs) are active in reducing levels of both cytoplasmic mRNAs and nuclear retained RNAs. Although ASO activity in the nucleus has been well demonstrated, the cytoplasmic activity of ASOs is less clear. Using kinetic and subcellular fractionation studies...

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Détails bibliographiques
Publié dans:Mol Ther
Auteurs principaux: Liang, Xue-Hai, Sun, Hong, Nichols, Joshua G., Crooke, Stanley T.
Format: Artigo
Langue:Inglês
Publié: American Society of Gene & Cell Therapy 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5589097/
https://ncbi.nlm.nih.gov/pubmed/28663102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymthe.2017.06.002
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