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RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus

RNase H1-dependent antisense oligonucleotides (ASOs) are active in reducing levels of both cytoplasmic mRNAs and nuclear retained RNAs. Although ASO activity in the nucleus has been well demonstrated, the cytoplasmic activity of ASOs is less clear. Using kinetic and subcellular fractionation studies...

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Publicat a:Mol Ther
Autors principals: Liang, Xue-Hai, Sun, Hong, Nichols, Joshua G., Crooke, Stanley T.
Format: Artigo
Idioma:Inglês
Publicat: American Society of Gene & Cell Therapy 2017
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5589097/
https://ncbi.nlm.nih.gov/pubmed/28663102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymthe.2017.06.002
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