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Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
Crosslinking and immunoprecipitation (CLIP) followed by high-throughput sequencing identifies the binding sites of RNA binding proteins on RNAs. The covalent RNA-amino acid adducts produced by UV irradiation can cause premature reverse transcription termination and deletions (referred to as crosslin...
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| Pubblicato in: | Methods |
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| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2017
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5582984/ https://ncbi.nlm.nih.gov/pubmed/28790018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.08.002 |
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