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CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins
Identification of in vivo direct RNA targets for RNA binding proteins (RBPs) provides critical insight into their regulatory activities and mechanisms. Recently, we described a methodology for enhanced crosslinking and immunoprecipitation followed by high-throughput sequencing (eCLIP-seq) using anti...
Shranjeno v:
| izdano v: | Methods |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2016
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5411315/ https://ncbi.nlm.nih.gov/pubmed/28003131 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.12.007 |
| Oznake: |
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