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Selective amplification in methotrexate-resistant mouse cells of an artificial dihydrofolate reductase transcription unit making use of cryptic splicing and polyadenylation sites.

We have constructed a recombinant ( pMOP ) which is based on the bacterial plasmid pML2 and contains bovine papilloma virus type 1 (BPV 1) sequences linked to an artificial mouse dihydrofolate reductase (DHFR) transcription unit. This unit consists of the SV40 early gene promoter, a complete DHFR co...

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Autore principale: Breathnach, R
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1984
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC557445/
https://ncbi.nlm.nih.gov/pubmed/6202514
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