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Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
Engineering and study of protein function by directed evolution has been limited by the requirement to introduce DNA libraries of defined size or to use global mutagenesis. Here, we develop a strategy to repurpose the somatic hypermutation machinery used in antibody affinity maturation to efficientl...
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| Publicado no: | Nat Methods |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5557288/ https://ncbi.nlm.nih.gov/pubmed/27798611 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4038 |
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