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Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
Engineering and study of protein function by directed evolution has been limited by the requirement to introduce DNA libraries of defined size or to use global mutagenesis. Here, we develop a strategy to repurpose the somatic hypermutation machinery used in antibody affinity maturation to efficientl...
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| Udgivet i: | Nat Methods |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5557288/ https://ncbi.nlm.nih.gov/pubmed/27798611 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4038 |
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