Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

Engineering and study of protein function by directed evolution has been limited by the requirement to introduce DNA libraries of defined size or to use global mutagenesis. Here, we develop a strategy to repurpose the somatic hypermutation machinery used in antibody affinity maturation to efficientl...

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Detaylı Bibliyografya
Yayımlandı:Nat Methods
Asıl Yazarlar: Hess, Gaelen T., Frésard, Laure, Han, Kyuho, Lee, Cameron H., Li, Amy, Cimprich, Karlene A., Montgomery, Stephen B., Bassik, Michael C.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2016
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5557288/
https://ncbi.nlm.nih.gov/pubmed/27798611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4038
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