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Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish
Although the CRISPR/Cas9 has been successfully applied in zebrafish, considerable variations in efficiency have been observed for different gRNAs. The workload and cost of zebrafish mutant screening is largely dependent on the mutation rate of injected embryos; therefore, selecting more effective gR...
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| Publié dans: | PLoS One |
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| Auteurs principaux: | , , , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Public Library of Science
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5553855/ https://ncbi.nlm.nih.gov/pubmed/28800611 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0182528 |
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